European Journal of Taxonomy 93: 1-16

http://dx.doi.org/10.5852/ejt.2014.93

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2014 • Nzigidahera B. & Jocque R.

Research article

urn:lsid:zoobank.org:pub:5560D3E8-0A4A-45EF-9108-8F9E23F5520F

On the genus Pachygnatha (Araneae, Tetragnathidae) in the Albertine

Rift of Burundi, with the description of three new species

Benoit NZIGIDAHERA 1 & Rudy JOCQUE 2

1 Institut National pour TEnvironnement et la Conservation de la Nature, B.P. 2757 Bujumbura, Burundi

2 Royal Museum for Central Africa, B-3080 Tervuren, Belgium

Email: [email protected] (corresponding author)

1 urn:lsid:zoobanlc.org:author:3112C6AF-BB9B-4CF7-B0A6-A709D2473A25

2 urn:lsid:zoobank.org:author:CF15016C-8CDl-4C9D-9021-44CA7DC7A5D5

Abstract. Three new species of Pachygnatha , P. bispiralis sp. nov., P. intermedia sp. nov. and P.

ventricosa sp. nov., are described from forest areas in western Burundi. The presence of P. procincta

Bosmans & Bosselaers, 1994 in Burundi confirms its very wide distribution spanning most of Africa.

Pachygnatha appears to be an important element of the afromontane spider fauna.

Keywords. Kibira National Park, Afromontane forest, new species, genus template, biodiversity hotspot.

Nzigidahera B. & Jocque R. 2014. On the genus Pachygnatha (Araneae, Tetragnathidae) in the Albertine Rift

of Burundi, with the description of three new species. European Journal of Taxonomy 93: 1-16. http://dx.doi.

org/10.5852/eit.2014,93

Introduction

Twelve species of Pachygnatha Sundevall, 1823 are known from tropical Africa (Platnick 2014).

Bosmans & Bosselaers (1994) gave an excellent overview of this fauna and not only described several

new species from Cameroon but also redescribed all known species except one, P. africana Strand, 1906

for which the types could not be traced, doubtless because they have been destroyed during World War II

(Renner 1988).

Of the twelve species, two are known from Central Africa, and more precisely from mountainous areas

in the Albertine Rift: Pachygnatha kiwuana Strand, 1913, and Pachygnatha ruanda Strand, 1913.

During three years (2003, 2004-2005, 2008) of field work along altitudinal gradients (793 to 2650 m)

in the afromontane forest of Kibira, miombo forest of Rumonge and circum-Guinean forest of Kigwena,

four species of Pachygnatha have been found. Only Pachygnatha procincta Bosmans & Bosselaers,

1994 has been previously described. It apparently has a wide distribution and was known from Cameroon

and Kenya. Three other species are here described as new.

Material and methods

Primary types are deposited in the Musee Royal de FAfrique Centrale, Tervuren, Belgium (MRAC),

whereas paratypes are divided among MRAC and the Institut National pour TEnvironnement et

1

European Journal of Taxonomy 93: 1-16 (2014)

la Conservation de la Nature, Bujumbura, Burundi (INECN). Specimens were observed, drawn and

measured with a WILD M 10 stereo microscope. Details of the female genitalia and male palps were

observed with a Zeiss Stemi 2000 strereo microscope.

Female genitalia were detached from the abdomen, cleared with methyl salicylate and temporarily

mounted in a mixture of methyl salicylate and cedulcol for examination. The same method was used

to observe details of the male palp. These structures were photographed with a Leica MZ16 stereo

microscope and subject to automontage with the Syncroscopy software. All measurements are in mm.

As with the illustrations, they were taken from the holotype and paratypes unless stated otherwise.

Coordinates are in decimal format. The distribution map fox Pachygnathaprocincta (Fig. 6) shows only

one spot in Burundi: it covers both the adjacent collection sites Kibira and Kigwena mentioned in this

paper and of which the former is the type locality of the three new species.

Abbreviations

ALE =

AME =

AW

Fe

INECN =

L

MOQ =

MRAC =

Mt

PA

PLE

PME =

PW

t

T

anterior lateral eyes

anterior median eyes

anterior width

femur

Institut National pour FEnvironnement et la Conservation de la Nature (Bujumbura, Burundi)

length

median ocular quadrangle

Musee Royal de FAfrique Centrale (Tervuren, Belgium)

metatarsus

patella

posterior lateral eyes

posterior median eyes

posterior width

tarsus

tibia

Results

Class Arachnida Cuvier, 1812

Order Araneae Clerck, 1757

Family Tetragnathidae Menge, 1866

Genus Pachygnatha Sundevall, 1823

Pachygnatha bispiralis sp. nov.

um:lsid:zoobank.org:act:E6CDD3F2-0E67-4520-A794-E5D48E37EFC8

Figs 1A-C, 2A-D

Diagnosis

Males are easily recognized by several unique features: the peculiar cymbium which is strongly

narrowed at the mid-point, the very long conductor protruding beyond the cymbium, the tibia with a

large ventrolateral apophysis and the very large, claw shaped, distal spur on the chelicerae.

Etymology

The specific name bispiralis refers to the broad embolus with longitudinal groove giving the impression

of a sclerite with two spirals.

2

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NZIGIDAHERAB. & JOCQUE R., New Pachygnatha species (Araneae, Tetragnathidae) from Burundi

Type material examined

Holotype

BURUNDI: 8, Kibira National Park, Rwegura, Mt Musumba, 02.87696°S, 029.49709°E, site 1,

mountain heather with Philippia benguelensis, 10 Mar. 2008, 2650 m, pitfalls, B. Nzigidahera (MRAC

226712).

Paratypes

BURUNDI: 2 88 , Kibira National Park, Rwegura, Mt Musumba, 02.86435°S, 029.49540°E, site 7, tea

plantation, 10 Sep. 2008, 2100 m, pitfalls, B. Nzigidahera (INECN); 1 8, same locality, 02.86435°S,

029.49546°E, site 6, forest with Carapa grandiflora and Polyscias fulva , 25 Mar. 2008, 2150 m (MRAC

226711); 1 8, 1 juv., same locality, 10 Mar. 2008 (MRAC 226730); 1 8, same locality, 02.86445°S,

029.49640°E, site 5, forest with Carapa grandiflora and Polyscias fulva , 25 Jul. 2008, 2252 m (MRAC

226722); 1 8 , same locality, 10 Apr. 2008 (MRAC 226724); 1 8 , same locality, 25 Feb. 2008 (MRAC

226731); 2 88 , same locality, 10 Dec. 2008 (MRAC 226732); 1 8 , same locality, 25 Dec. 2008 (MRAC

226725); 1 8 , same locality, 15 Mar. 2008 (MRAC 226719); 3 88 , same locality, 26 Jan. 2008 (MRAC

226721); 1 8, same locality, 02.87149°S, 029.49641°E, site 3, forest with Hagenia abyssinica, 25 Apr.

2008, 2444 m (MRAC 226727); 1 8 , same locality, 26 Jan. 2008 (MRAC 226715); 1 8 , same locality,

10 Mar. 2008 (MRAC 226716); 1 8, same locality, 25 Nov. 2008 (MRAC 226723); 3 88, same

locality, 25 Feb. 2008 (MRAC 226720); 1 8 , same locality, 10 Nov. 2008 (INECN); 1 8 , same locality,

02.87060°S, 029.49669°E, site 2, forest with Hagenia abyssinica , 25 Apr. 2008, 2548 m (INECN);

1 8, same locality, 02.87696°S, 029.49709°E, site 1, forest mountain heather, Phillipia benguelensis,

25 Dec. 2008, 2650 m (MRAC 226717); 2 88, same locality, 10 Nov. 2008 (MRAC 226729); 1 8,

same locality, 25 Oct. 2008 (MRAC 226726); 1 8, same locality, 25 Mar. 2008 (MRAC 226564); 1 8,

same locality, 25 Jun. 2008 (INECN); 1 8, same locality, 10 Sep. 2008 (INECN); 1 8, same locality,

25 Feb. 2008 (INECN); 1 8, same locality, 0289891°S, 02950405°E, site 1, tea plantation, 15 May

2005, 1970 m (INECN); 1 8, Kibira National Park, Rusarenda Forest, 03.14228°S, 029.56235°E, forest

with Neoboutonia macrocalyx and Myrianthus holstii , 12 Feb. 2003, 2237 m, pitfalls, B. Nzigidahera

(INECN); 1 8, Kigwena Nature Reserve, Kigwena Forest, 02.89891°S, 029.50405°E, forest with

Newtonia buchananii and Albizia zygia, 5 Jan. 2004, 793 m, pitfalls, B. Nzigidahera (INECN).

Description

Male

Holotype, Figs 1A-C, 2A-D.

Measurements. Total length 2.70, carapace 1.20 long, 1.08 wide.

Carapace. Pale brown, with two lateral dark brown bands; prominent in anterior part.

Eyes. AME: 0.12, ALE: 0.12, PLE: 0.12, PME: 0.20, AME-AME: 0.02, PME-PME: 0.02, AME-ALE:

0.04, PME-PLE: 0.06, ALE-PLE: 0.00, MOQ: AW: 0.26, PW: 0.32, L: 0.32.

Chelicerae. (Fig. 2C-D). Four promarginal teeth, four retromarginal teeth, large claw shaped prolateral

spur with broad base and sharp, curved tip.

Sternum. Brownish grey with some setae, distal margin concave; intercoxal sclerites present.

Legs. Yellowish brown; one distal dorsal spine on patellae; no further spines.

Abdomen. Yellowish grey with irregular, connected dark grey spots in almost three longitudinal lines,

mixed with silvery spots. Spinnerets pale yellow.

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European Journal of Taxonomy 93: 1-16 (2014)

Fig. 1 . A-C. Pachygnatha bispiralis sp. nov. A. Male habitus, dorsal view. B. Male habitus, ventral

view. C. Male habitus, lateral view. D-I. Pachygnatha intermedia sp. nov. D. Male habitus, dorsal view.

E. Male habitus, ventral view. F. Male habitus, lateral view. G. Female habitus, dorsal view. H. Female

habitus, ventral view. I. Female habitus, lateral view. J-O. Pachygnatha ventricosa sp. nov. J. Male

habitus, dorsal view. K. Male habitus, ventral view. L. Male habitus, lateral view. M. Female habitus,

dorsal view. N. Female habitus, ventral view. O. Female habitus, lateral view. Scale bars = 2 mm.

4

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NZIGIDAHERAB. & JOCQUE R., New Pachygnatha species (Araneae, Tetragnathidae) from Burundi

Palp. (Fig. 2A-B). Sclerotised tibial apophysis very broad, large blade-shape retrolaterally high, ventrally

short. Paracymbium long, straight with some long hairs at the sharp, slightly curved tip. Cymbium very

broad at base, thin in middle and wider towards tip. Embolus long, running along tegulum and beyond

to tip of cymbium, supported by conductor.

Leg measurements

Leg

Fe

Pa

T

Mt

t

Total

I

1.64

0.48

1.76

1.44

0.80

6.12

II

1.60

0.44

1.96

1.28

1.76

7.04

III

1.20

0.40

0.92

0.88

0.48

3.88

IV

1.48

0.40

1.40

1.28

0.64

5.20

Female

Unknown.

Distribution

Known from Kibira National Park and Kigwena Nature Reserve in Burundi.

Fig. 2. Pachygnatha bispiralis sp. nov. A. Male left palp, dorsal view. B. Male palp, retrolateral view.

C. Male right chelicera, frontal view. D. Male right chelicera, ventral view. Scale bars = 0.1 mm

5

European Journal of Taxonomy 93 : 1-16 ( 2014 )

Pachygnatha intermedia sp. nov.

urn:lsid:zoobank.org:act:AEl 50829-001F-4063-94A1-86FDA88B309D

Figs ID—I, 3A-H, 5A

Diagnosis

Males are recognized by details of the palp: the cymbium is unique in having a central membranous area

dividing it into a proximal and a distal part, the latter provided with a short, thin prong; the paracymbium

is broad at its base with a retrolateral swelling and a triangular prolateral extension, the distal part is

slender and tapered to a point; the tibia has a well developed truncate dorsal apophysis. The female is

recognized by the rectangular sclerotised areas in front and behind the genital opening.

P. intermedia sp. nov. is closely related to P. okuensis Bosmans & Bosselaers, 1994. The males share the

distal paracymbial excavation, the shape of the cheliceral fang and the lozenge shaped paracymbium.

Both the latter differ in details of the shape: in P. intermedia the triangular extension of the cheliceral

fang is much more pronounced and the distal prong of the paracymbium is much more slender and

longer; the excavation of the cymbium is subdistal whereas it is at the distal tip of P. okuensis. The

female has a cheliceral spur on the promargin of the chelicerae. With P. goedeli Bosmans & Bosselaers,

1994 it shares the paracymbium with long narrow distal extension and the small cymbial distal prong

but details of these sclerites are clearly different.

Etymology

The specific name intermedia refers to shape of the paracymbium which is of median size in between the

very broad paracymbium of Pachygnatha ventricosa sp. nov. and the narrow one of P. bispiralis sp. nov.

Type material examined

Holotype

BURUNDI: 3 , Kibira National Park, Rwegura, Mt Musumba, 02.87149°S, 029.49641°E, site 3, forest

with Hagenia abyssinica, 10 Jul. 2008, 2444 m, pitfalls, B. Nzigidahera (MRAC 226552).

Paratypes

BURUNDI: 1 3 , Kibira National Park, Rwegura, Mt Musumba (same locality for all paratypes),

02.86435°S, 029.49540°E, site 7, tea plantation, 10 Dec. 2008, 2100 m, pitfalls, B. Nzigidahera (MRAC

226772); 1 3 , 25 Feb. 2008 (INECN); 1 $, 25 Oct. 2008 (INECN); 1 3 , 02.86435°S, 029.49546°E, site

6, forest with Carapa grandiflora and Polyscias fulva , 25 Feb. 2008, 2150 m (MRAC 226558); 3 33 ,

25 Jul. 2008(MRAC226562); 1 3 , lONov. 2008(INECN);3 ??, 02.86445°S, 029.49640°E, site5, forest

with Carapa grandiflora and Polyscias fulva , 25 Apr. 2008, 2252 m (MRAC 226747); 1 3 , 25 Jul. 2008

(MRAC 226573); 1 ?, 10 May 2008 (MRAC 226528); 1 3 , 10 Jun. 2008 (MRAC 226551); 1 3 , 10 Jul.

2008 (MRAC 226548); 1 3, 02.86455°S, 029.50427°E, site 4, forest with Macaranga neomildbraediana

and Polyscias fulva, 10 Oct. 2008, 2352 m (MRAC 226735); 2 33 , 25 Mar. 2008 (MRAC 226554); 1 $,

25 May 2008 (MRAC 226543); 2 $$, 25 Sep. 2008 (MRAC 226535); 2 $$, 25 Mar. 2008 (MRAC

226534); 2 33 , 10 Jan. 2008 (MRAC 226555); 1 3 , 25 Aug. 2008 (MRAC 226547); 2 33 , 25 Feb.

2008 (MRAC 226571); 1 ?, 25 Sep. 2008 (MRAC 226527); 1 3 , 10 Dec. 2008 (INECN); 1 3 , 25 Apr.

2008 (INECN); 2 33 , 10 Aug. 2008 (INECN); 1 3 , 25 Oct. 2008 (INECN); 1 3 , 25 Jun. 2008 (INECN);

3 ??, 25 Jul. 2008 (INECN); 1 ?, 25 Dec. 2008 (INECN); 1 ?, 10 Sep. 2008 (INECN); 2 $$, 10 Apr.

2008 (INECN); 3 33 , 02.87149°S, 029.49641°E, site 3, forest with Hagenia abyssinica , 25 Nov. 2008,

2444 m (MRAC 226566); 1 & 10 Jul. 2008 (MRAC 226552); 1 ?, 25 Dec. 2008 (MRAC 226544);

4 33 , 2 juvs, 26 Jan. 2008 (MRAC 226549); 1 3 , 10 Sep. 2008 (MRAC 226569); 3 33 , 25 Feb. 2008

(MRAC 226561); 1 $, 10 Apr. 2008 (MRAC 226524); 1 3 , 25 Jun. 2008 (MRAC 226563); 1 3 , 10 Dec.

2008 (MRAC 226556); 1 3 , 10 Oct. 2008 (INECN); 2 10 Feb. 2008 (INECN); 1 $, 26 Jan. 2008

6

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NZIGIDAHERAB. & JOCQUE R., New Pachygnatha species (Araneae, Tetragnathidae) from Burundi

(INECN); 4 $ $, 10 Mar. 2008 (INECN); 1 8, 10 Feb. 2008 (INECN); 2 88, 02.87060°S, 029.49669°E,

site 2, forest with Hagenia abyssinica, 26 Jan. 2008, 2548 m (MRAC 226756); 2 88, 25 Jul. 2008

(MRAC 226553); 1 8, 25 Dec. 2008 (MRAC 226568); 2 88, 1 juv., 25 Feb. 2008 (MRAC 226572);

1 8, 10 Aug. 2008 (INECN); 2 $$, 02.87696°S, 029.49709°E, site 1, forest mountain heather, Phillipia

benguelensis, 10 Mar. 2008, 2650 m (MRAC 226539); 1 8, 10 May 2008 (MRAC 226567); 1 8, 10 Apr.

2008 (MRAC 226559); 1 8, 10 Jun. 2008 (MRAC 226557); 3 $$,25 Dec. 2008 (MRAC 226540);

1 8, 25 Apr. 2008 (MRAC 226548); 1 8, 10 Nov. 2008 (MRAC 226560); 4 88, 25 Feb. 2008 (MRAC

226550); 1 8, 25 May 2008 (MRAC 226565); 2 $ $, 10 Sep. 2008 (MRAC 226545); 4 88, 25 Dec. 2008

(INECN); 2 88, 25 Mar. 2008 (INECN); 2 88, 10 Mar. 2008 (INECN); 1 8, 25 Jul. 2008 (INECN); 1 8,

25 Sep. 2008 (INECN); 2 $ $, 25 Mar. 2008 (INECN); 3 $ $, 25 Feb. 2008 (INECN).

Description

Male

Holotype, Figs 1D-F, 3A-D.

Measurements. Total length 3.80, carapace 1.90 long, 1.60 wide.

Carapace. Brown, with prominent anterior part delimitated by dark brown lines converging in fovea in

Y-shape.

Eyes. AME: 0.14, ALE: 0.14, PLE: 0.12, PME: 0.22, AME-AME: 0.04, PME-PME < 0.02, AME-ALE:

0.12, PME-PLE: 0.14, ALE-PLE: 0.00, MOQ: AW: 0.36, PW: 0.40, L: 0.44.

Chelicerae. (Fig. 3C-D). Three promarginal teeth, four retromarginal teeth, with oval spur at tip.

Cheliceral fang curved with pronounced triangular extension in the middle.

Sternum. Brown, with a sharp sclerotised point at each end of the slightly concave anterior margin;

intercoxal extensions present.

Legs. Pale yellowish brown with some vague grey spots near femur-patella and tibia-metatarsus

articulations; one dorsal distal spine on patellae; no further spines.

Abdomen. Dorsum pale grey with irregular, connected dark grey spots in almost three longitudinal

lines, raising a pale area in anterior part; venter pale grey with silvery spots in four longitudinal lines.

Spinnerets yellowish grey.

Palp. (Fig. 3A-B). Dorsal tibial apophysis rectangular, retrolateral tibial apophysis oval, sclerotised,

with setae, ventral tibial apophysis broad, sclerotised, blade shaped. Paracymbium with lozenge-shaped

base and long, slender, distal prong surpassing the tegulum. Cymbium with remarkable membranous

central part, with deep subdistal excavation at the tip, delimiting a thin lateral prong with a few setae.

Embolus long, broad at the base, thin in middle, slightly widened and with flat, bifid tip; conductor with

large, strongly sclerotised, distal part.

Leg measurements

Leg

Fe

Pa

T

Mt

t

Total

I

2.24

0.60

2.28

1.80

1.80

8.72

II

2.12

0.68

2.00

1.60

1.60

8.00

III

1.60

0.60

1.24

1.20

1.20

5.84

IV

2.20

0.52

2.00

1.84

1.84

8.40

7

European Journal of Taxonomy 93 : 1-16 ( 2014 )

Fig. 3. Pachygnatha intermedia sp. nov. A. Male palp, ventral view. B. Male palp, retrolateral view.

C. Male chelicera, frontal view. D. Male chelicera, ventral view. E. Female chelicera, ventral view.

F. Female chelicera, frontal view. G. Female abdomen, ventral view. H. Female external genitalia,

ventral view. Scale bars: A-F, H = 0.1 mm; G = 1 mm

8

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NZIGIDAHERAB. & JOCQUE R., New Pachygnatha species (Araneae, Tetragnathidae) from Burundi

Female

Paratype, MRAC 226544, Figs 1G-I, 3E-H. Very similar to male.

Measurements. Total length 4.80. Carapace. 1.92 long, 1.50 wide.

Eyes. AME: 0.14, ALE: 0.16, PME: 0.24, PLE: 014, AME-AME: 0.06, PME-PME < 0.02, ALE-PLE:

0.00, AME-ALE: 0.10, PME-PLE: 0.12; MOQ: AW: 0.44, PW: 0.32, L: 0.44.

Chelicerae. (Fig. 3E-F). Yellowish brown, 3 teeth on promargin and basal spur, 3 teeth on retromargin.

External genitalia. (Figs 3G-H, 5A). With rectangular sclerotised areas in front of and be hin d the

genital opening.

Leg measurements

Leg

Fe

Pa

T

Mt

t

Total

I

1.84

0.68

1.80

1.44

0.80

6.50

II

1.80

0.68

1.60

1.40

0.76

6.24

III

1.36

0.56

1.00

1.00

0.52

4.44

IV

1.96

0.52

1.68

1.60

0.64

6.40

Other material examined

BURUNDI: 3 $$, 2 $ §, Kibira National Park, Rwegura Forest, 02.89435°S, 029.50078°E, forest with

Maracanga neomildbreadiana and Xymalos monospora , 19 Mar. 2003, 2223 m, pitfalls, B. Nzigidahera

(INECN); 1 $, same locality, 23 May 2003 (INECN); 1 same locality, 27 Dec. 2003 (INECN); 2 <3S,

5 5$, same locality, 13 Sep. 2003 (INECN); 1 S, same locality, 14 Jan. 2004 (INECN); 1 §, Kibira

National Park, Rusarenda Forest, 03.14228°S, 029.56235°E, forest with Neobontonia macrocalyx and

Myrianthus holstii , 12 Feb. 2003, 2237 m, pitfalls, B. Nzigidahera (INECN); 3 1 $, 1 juv., same

locality, 12 Feb. 2003 (INECN); 1 S, 1 1 juv., same locality, 21 Mar. 2003 (INECN); 1 $, Kibira

National Park, Bugarama Forest, 03.26537°S, 029.54921°E, forest with Symphonia globulifera and

Parinari holstii , 28 Mar. 2003, 2202 m, pitfalls, B. Nzigidahera (INECN); 1 <$, same locality, 14 Oct.

2003 (INECN); 1 S, same locality, 15 Jul. 2003 (INECN); 1 (?, 1 juv., same locality, 14 Oct. 2003

(INECN); 1 same locality, 30 Jul. 2003 (INECN); 3 SS, Kigwena Nature Reserve, Kigwena Forest,

02.89891°S, 029.50405°E, forest with Newtonia buchananii and Albizia zygia, 5 Jan. 2004, 793 m,

pitfalls, B. Nzigidahera (INECN).

Distribution

Known from Kibira National Park and Kigwena Nature Reserve in Burundi.

Pachygnatha ventricosa sp. nov.

urn:lsid:zoobank.org:act:356C3177-C153-47D7-865B-7FDFlCE821El

Figs 1J-0, 4A-H, 5B

Diagnosis

Males of P. ventricosa sp. nov. are recognized by details of the palp: the paracymbium has a broad

basal part with semicircular retrolateral swelling and small triangular prolateral extension; the cymbium

has two parts: a broad basal part, divided from the parallel-sided distal part by a deep, membranous

indentation. This resembles that of P. bispiralis sp. nov. , but the narrow central part of the cymbium is

much longer and not membranous. In Pachygnatha intermedia sp. nov. there is a central membranous

cymbial area but the shape of that sclerite is very different. This is somewhat similar to that of P.

9

European Journal of Taxonomy 93: 1-16 (2014)

kiwuana Strand, 1913 but the distal part of the paracymbium is much longer and well separated from the

basal part in P. ventricosa sp. nov. Females are characterized by external genitalia with a sclerotised and

transverse expansion of the upper lip forming a rectangular shape.

Etymology

The specific name ventricosa refers to the inflated, belly-shaped lateral part of the paracymbium on the

male palp.

Type material examined

Holotype

BURUNDI: 3, Kibira National Park, Rwegura, Mt Musumba, 02.86445°S, 029.49640°E, site 5, forest

with Carapa grandiflora and Polyscias fulva, 10 Jul. 2008, 2252 m, pitfalls, B. Nzigidahera (MRAC

226777).

Para types

BURUNDI: 1 3, Kibira National Park, Rwegura, Mt Musumba (same locality for all paratypes),

02.86435°S, 029.49540°E, site 7, tea plantation, 26 Jan. 2008,2100 m, pitfalls, B. Nzigidahera (INECN);

1 3, 10 Sep. 2008 (INECN); 1 $, 25 Apr. 2008 (MRAC 226536); 1 ?, 02.86435°S, 029.49546°E, site 6,

forest with Carapa grandiflora and Polyscias fulva, 26 Jan. 2008,2150m (MRAC 226766); I f, 25 Dec.

2008 (MRAC 226768); 1 ?, 10 May 2008 (MRAC 226736); 1 ?, 25 Dec. 2008 (MRAC 226748); 1 ?,

10 Apr. 2008 (MRAC 226531); 4 25 Aug. 2008 (INECN); 4 ??, 10 Jan. 2008 (INECN); 2

2 juvs: 25 Nov. 2008 (INECN); 1 $, 25 Sep. 2008 (INECN); 3 $?, 10 Aug. 2008 (INECN); 1 3, 2 juvs,

02.86445°S, 029.49640°E, site 5, forest with Carapa grandiflora and Polyscias fulva, 10 Aug. 2008,

2252 m (MRAC 227427); 1 ?, 2 juvs, 25 Jun. 2008 (MRAC 226775); 1 3, 10 Sep. 2008 (MRAC

226771); 1 ?, 1 juv., 10 Apr. 2008 (MRAC 226763); 2 33, 25 Jul. 2008 (MRAC 226758); 1 $, 25 Mar.

2008 (MRAC 226754); 2 33, 3 ??, 1 juv., 25 Feb. 2008 (MRAC 226734); 2 $$, ljuv.,25Aug. 2008

(MRAC 226760); 1 ?, 10 Sep. 2008 (MRAC 336532); 2 33, 25 Sep. 2008 (INECN); 1 $, 26 Jan. 2008

(INECN); 1 $, 25 Jul. 2008 (INECN); 1 $, 10 Feb. 2008 (INECN); 2 33, 02.86455°S, 029.50427°E,

site 4, forest with Macaranga neomildbraediana and Polyscias fulva, 10 Jan. 2008, 2352 m (MRAC

226757); 1 S, 26 Jan. 2008 (MRAC 226761); 1 $, 25 May 2008 (MRAC 226750); 1 $, 10 Sep. 2008

(MRAC 226752); 2 S3, 23 Jun. 2008 (MRAC 226753); 1 3, 25 Nov. 2008 (MRAC 226773); 2 33,

25 Mar. 2008 (MRAC 226764); 1 3, 10 Oct. 2008 (MRAC 226574); 1 3, 10 Oct. 2008 (INECN); 1 ?,

25 Dec. 2008 (INECN); 1 5, 10 Jul. 2008 (INECN); 5 ?10 Jan. 2009 (INECN); 7 $ ?, 25 Aug. 2008

(INECN); 2 5?, 10 Dec. 2008 (INECN); 8 ?$, 26 Jan. 2008 (INECN); 2 ?$, 10 Nov. 2008 (INECN);

4 25 Oct. 2008 (INECN); 2 $?, 25 Nov. 2008 (INECN); 2 33, 02.87149°S, 029.49641°E, site 3,

forest with Hagenia abyssinica, 25 Oct. 2008, 2444 m (MRAC 226770); 6 5?, 26 Jan. 2008 (MRAC

226762); 2 33, 10 Sep. 2008 (MRAC 226774); 1 $, 25 Apr. 2008 (MRAC 226769); 3 33, 26 Jan. 2008

(MRAC 226733); 1 ?, 10 Jun. 2008 (MRAC 226751); 1 ?, 10 Mar. 2008 (MRAC 226737); 1 3, 1 ?,

3 juvs, 25 Feb. 2008 (MRAC 226739); 1 $, 25 Nov. 2008 (MRAC 226740); 1 ?, 10 Dec. 2008 (MRAC

226537); 1 ?, 10 May 2008 (MRAC 226523); 1 $, 25 Nov. 2008 (MRAC 226529); 1 ?, 10 Apr. 2008

(INECN); 1 3, 10 Mar. 2008 (INECN); 1 3, 25 Jul. 2008 (INECN); 2 ??, 10 Apr. 2008 (INECN);

2 25 Aug. 2008 (INECN); 1 $, 25 Sep. 2008 (INECN); 10 ?, 5 juvs, 25 Feb. 2008 (INECN); 2

10 Sep. 2008 (INECN); 1 ?, 2 juvs, 10 Aug. 2008 (INECN); 2 $?, 25 Jan. 2008 (INECN); 1 $, 10 Nov.

2008 (INECN); 2 33, 02.87060°S, 029.49669°E, site 2, forest with Hagenia abyssinica, 25 Aug. 2008,

2548 m (MRAC 226749); 1 3, 10 Sep. 2008 (MRAC 226767); 1 ?, 25 Sep. 2008 (MRAC 226742);

1 3, 10 Sep. 2008 (MRAC 226745); 1 ?, 25 Apr. 2008 (MRAC 226755); 1 $, 25 Dec. 2008 (MRAC

226542); 1 3, 10 Dec. 2008 (MRAC 226570); 1 $, 10 Jan. 2008 (MRAC 226526); 1 3, 2 juvs, 25 Jul.

2008 (INECN); 1 $, 1 juv., 10 Feb. 2008 (INECN); 1 25 Mar. 2008 (INECN); 2 33, 2 juv., 25 Feb.

2008 (INECN); 2 33, 4 ? 3 juvs, 02.87696°S, 029.49709°E, site 1, forest mountain heather, Phillipia

benguelensis, 10 Mar. 2008,2650 m (MRAC 226743); 2 33, 2 juvs,10 Sep. 2008 (MRAC 226776); 1 3,

10

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NZIGIDAHERAB. & JOCQUE R., New Pachygnatha species (Araneae, Tetragnathidae) from Burundi

Fig. 4. Pachygnatha ventricosa sp. nov. A. Male palp, ventral view. B. Male palp, retrolateral view.

C. Male chelicera, frontal view. D. Male chelicera, ventral view. E. Female chelicera, ventral view.

F. Female chelicera, frontal view. G. Female abdomen, ventral view. H. Female external genitalia,

ventral view. Scale bars: AF, H = 0.1 mm; G = 1 min

11

European Journal of Taxonomy 93: 1-16 (2014)

10 Jun. 2008 (MRAC 226746); 1 ?, 10 Sep. 2008 (MRAC 226759); 3 ? 5 juvs, 25 Mar. 2008 (MRAC

226778); 1 3, 10 Aug. 2008 (MRAC 226765); 2 $$, 25 Feb. 2008 (MRAC 226744); 1 3, 10 Mar.

2008 (MRAC 226741); 1 3, 10 Mar. 2008 (MRAC 226712); 2 $$, 10 Mar. 2008 (MRAC 226530);

1 ?, 25 Nov. 2008 (MRAC 226541); 1 3, 10 Oct. 2008 (INECN); 1 3, 10 Nov. 2008 (INECN); 2

25 Feb. 2008 (INECN); 2 $ $, 25 Aug. 2008 (INECN); 6 ? $, 25 Feb. 2008 (INECN); 1 $, 25 Jun. 2008

(INECN); 2 $$, 10 Jan. 2009 (INECN); 2 $ $, 25 Mar. 2008 (INECN); 2 $$, 10 Nov. 2008 (INECN);

1 ?, 10 Jun. 2008 (INECN); 1 $, 10 Jul. 2008 (INECN); 2 $$, 25 Oct. 2008 (INECN); 2 1 juv.,

25 Jul. 2008 (INECN); 1 3, 10 Jul. 2008 (MRAC 226738).

Description

Male

Holotype, Figs 1J-L, 4A-D.

Measurements. Total length 3.47, carapace 1.67 long, 1.34 wide.

Carapace. Pale brown, prominent anteriorly, with a mediodorsal longitudinal dark brown band and two

lateral margin dark brown bands.

Eyes. AME: 0.14, ALE 0.12, PLE: 0.12, PME 0.20, AME-AME: 0.04, PME-PME< 0.02, AME-ALE:

0.10, PME-PLE: 0.12, ALE-PLE: 0.00, MOQ: AW: 0.32, PW: 0.38, L: 0.34.

Chelicerae. (Fig. 4C-D). Yellowish brown, three promarginal teeth, three retromarginal teeth, an

additional, promarginal cylindrical spur and a sharp apical spur.

Sternum. Yellowish brown with a sharp sclerotised point at each end of the straight anterior margin;

intercoxal sclerites present. Labium brown.

Legs. Yellowish brown; one thin slightly recurved dorsal distal spine on patellae; no further spines.

Abdomen. Dorsum pale grey with irregular, connected dark grey spots in almost two longitudinal

lines mixed with silvery spots; venter yellowish grey, convex grey line and two silvery spots in two

longitudinal lines. Spinnerets pale yellowish brown.

Fig. 5. A. Pachygnatha intermedia sp. nov. Female genitalia, cleared, ventral view. B. Pachygnatha

ventricosa sp. nov. Female genitalia, cleared, ventral view. Scale bars = 0.1 mm.

12

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NZIGIDAHERAB. & JOCQUE R., New Pachygnatha species (Araneae, Tetragnathidae) from Burundi

Palp. (Fig. 4A-B). Retrolateral tibial apophysis sharp at tip, ventral tibial apophysis broad, blade-shaped.

Paracymbium long, broad on one side towards the base, with small horn to almost middle part of other

side. Embolus long, running spirally to the cymbium tip, supported by conductor.

Leg measurements

Leg

Fe

Pa

T

Mt

t

Total

I

2.28

0.60

0.40

2.00

1.00

6.28

II

2.08

0.60

2.12

1.80

0.80

7.4

III

1.36

0.52

1.20

1.08

0.52

4.68

IV

2.40

0.48

1.76

1.76

0.68

7.08

Female

Paratype, MRAC 226536, Figs 1M-0, 4E-H, 5B. Very similar to male.

Measurements. Total length 4.32. Carapace 1.72 long, 1.36 wide.

Eyes. AME: 0.14, ALE: 0.12, PME: 0.20, PLE: 012, AME-AME: 0.06, PME-PME: 0.02, ALE-PLE:

0.00, AME-ALE: 0.10, PME-PLE: 0.12; MOQ: AW: 0.40, PW: 0.32, L: 0.40.

Chelicerae. (Fig. 4E-F). Yellowish brown, 3 promarginal teeth, 3 retromarginal teeth, both margins

with short, sharp distal spur.

Legs. With some vague grey spots on articulations of femora and patella and the tip of tibia; one spine

on patella.

External genitalia. (Figs 4G-H, 5B). A simple, horizontal slit with an inferior part highly sclerotised

laterally, superior one blade-shape and connected to inferior one by hyaline extension in two extremities;

a sclerotised fold along the superior part.

Leg measurements

Legs

Fe

Pa

T

Mt

t

Total

I

2.40

0.68

2.80

2.12

1.00

9.00

II

2.20

0.68

2.16

1.80

0.88

7.72

III

F48

0.52

1.16

1.12

0.56

4.84

IV

2.12

0.48

1.80

1.72

0.52

6.64

Other material examined

BURUNDI: 1 §, Kibira National Park, Rwegura, foret de Rwegura, 02.89891°S, 29.50405°E, site 1, tea

plantation, 30 Sep. 2004, 1970 m, pitfalls, B. Nzigidahera (INECN); 1 S, same locality, 28 Feb. 2005

(MRAC 227618); 2 $ §, 1 juv., same locality, 02.89565°S, 029.50345°E, site 2, forest with Polysciasfulva

and Macaranga neomildbraediana, 30 Sep. 2004, 2120 m (INECN); 2 5$, same locality, 0289562°S,

02950221°E, site 3, forest with Polyscias fulva and Hagenia abyss mica, 15 Mar. 2005, 2160 m (MRAC

227616); 1 §, same locality, 02.90249°S, 029.51345°E, forest with Macaranga' neomildbreadiana and

Xymalos monospora, 26 Jul. 2003, 2223 m (INECN).

Distribution

Known only from the type locality.

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European Journal of Taxonomy 93: 1-16 (2014)

Pachygnatha procincta Bosmans & Bosselaers, 1994

Fig. 6

Material examined

BURUNDI: 1 Kibira National Park, Rwegura, Mt. Musumba, 02.86°S 29.50°E, site 7, tea plantation,

10 Dec. 2008, 2100 m, B. Nzigidahera (MRAC 226772).

Remarks

The specimen was compared to the holotype from Cameroon (MRAC 202728) and to the specimens

from two localities in Kenya (MRAC 111873, MRAC 112015), that were attributed to the same species

by Bosmans & Bosselaers (1994). Reexamination confirms the close resemblance between all these

specimens in which the males are characterized by the shape of the paracymbium. This sclerite has a

very typical comma shaped bald retrolateral area. The cheliceral dentition of the specimens is virtually

identical. The Burundian specimen has a slightly different colouration in the sense that it is paler and less

orange than the other specimens. The presently known distribution is shown in Fig. 6.

Discussion

This study is a result of the intense sampling campaign of ground spiders carried out in the forest

ecosystems of Western Burundi during several years (2003, 2005, 2008). Pachygnatha ventricosa

sp. nov. was found only in the afromontane forest of the Kibira National Park whereas Pachygnatha

bispiralis sp. nov. and P. intermedia sp. nov. were collected there and in the circum-Guinean forest of

Kigwena at lower altitude. According to our study, 4 species of Pachygnatha are found in this small part

of the Albertine Rift which corroborates the conclusion of Nzigidahera & Jocque (2009). They observed

Fig. 6. Distribution map of Pachygnatha procincta Bosmans & Bosselaers, 1994.

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NZIGIDAHERAB. & JOCQUE R., New Pachygnatha species (Araneae, Tetragnathidae) from Burundi

Table 1 . Altitude distribution of Pachygnatha Sundevall, 1823.

Species

Authorship

Country

Altitude

atromarginata

Bosnians & Bosselaers, 1994

Cameroon

1600

bispiralis

Nzigidahera & Jocque sp. nov.

Burundi

793-2650

goedeli

Bosmans & Bosselaers, 1994

Cameroon

>2200

hexatracheata

Bosmans & Bosselaers, 1994

Cameroon

> 1300

intermedia

Nzigidahera & Jocque sp. nov.

Burundi

793-2650

jansseni

Bosmans & Bosselaers, 1994

Cameroon

> 1200

kiwuana

Strand, 1913

Congo

> 1463

lelenpi

Lawrence, 1952

Cameroon

> 1200

longipes

Simon, 1894

Madagascar

sea level

mucronata

Tullgren, 1910

Tanzania

>2000

oknensis

Bosmans & Bosselaers, 1994

Cameroon

>2400

opdeweerdtae

Bosmans & Bosselaers, 1994

Cameroon

>2200

palmquisti

Tullgren, 1910

Tanzania

>2300

procincta

Bosmans & Bosselaers, 1994

Cameroon

>2100

ruanda

Strand, 1913

Rwanda

1800

ventricosa

Nzigidahera & Jocque sp. nov.

Burundi

1970-2650

zappa

Bosmans & Bosselaers, 1994

Cameroon

1900

that this Central African mountain range, recognized as a hot spot for many animal groups, also harbors

a high diversity of spiders often belonging to a particular genus which prompted the term ‘species

swarm’. In their study on the gnaphosid genus Zelotibia Russell-Smith & Murphy, 2005, Russell-

Smith & Murphy (2005) found that no less than 19 species are known from the area. This is therefore the

first African area for which there is evidence from the spider fauna that it deserves the epithet “hot spot”.

Other recent papers on the fauna of the area pointing in the same direction are those on the gnaphosid

Zelowan Murphy & Russell-Smith, 2010 (Murphy & Russell-Smith 2010), on zodariids (Nzigidahera

& Jocque 2010a, 2010b) and linyphiids (Nzigidahera & Jocque 2014). These studies also suggest that

the Albertine Rift is far from completely inventoried and several papers concerning its spider fauna are

in preparation.

The genus Pachygnatha has a remarkable distribution on the African continent. All the species have

been found above 1200 m (see Table 1) apart from a few specimens of the new species P. bispiralis

and P. intermedia , which are common around 2000 m and higher but were also collected near 800 m.

Pachygnatha thus appears to be a common element of the Afromontane spider fauna but had not yet

been recognized as such. Moreover, it is a typical case of a template genus with very stable somatic

characters but with a large range of secondary sexual characters, more precisely of the male palp and

the dentition of the chelicerae. A more extensive study of the genus may therefore yield insights into the

evolution of secondary sexual characters and their link with biogeographical distribution.

Acknowledgements

ABIC (African Biodiversity Information Centre) and GBIF are thanked for several travel grants to (BN).

We are indebted to the Royal Museum for Central Africa for accommodation for BN during his studies

in Belgium. We thank also Alain Reygel, who made the drawings with his usual skill. Jan Bosselaers is

thanked for his comments on a draft of this paper.

15

European Journal of Taxonomy 93: 1-16 (2014)

References

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(Araneae, Tetragnathidae), with a revision of the Afrotropical species. Zoologica Scripta 23 (4): 325-

352.

Murphy J.A. & Russell-Smith A. 2010. Zelowan, a new genus of African zelotine ground spiders

(Araneae: Gnaphosidae). Journal of afrotropical Zoology 6: 59-82.

Nzigidahera B. & Jocque R. 2009. An update of Zelotibia (Araneae, Gnaphosidae), a spider genus with

a species swarm in the Arbertine Rift. ZooKeys 13: 1-28. http://dx.doi.org/10.3897/zookevs. 13.145

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distribution of the genus into central Africa. Zootaxa 2578: 62-68.

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the description of three new species from the Albertine Rift. European Journal of Taxonomy 77: 1-18.

http://dx.doi.org/10.5852/ejt.2014.77

Platnick N.I. 2014. The world spider catalog. Version 10.0. American Museum of Natural History.

Available from http://research.amnh.org/iz/spiders/catalog/ [accessed 20 Jan. 2014]

Renner F. 1988. Liste der im Krieg vernichteten Typen des Koniglichen Naturalienlcabinetts in Stuttgart.

In: Haupt J. (ed.) XI Europdisches Arachnologisches Colloquium : 319-329. TUB-Dokumentation

Kongresse und Tagungen 38, Technische Universitat Berlin, Berlin.

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Manuscript received: 18 April 2014

Manuscript accepted: 16 June 2014

Published on: 13 August 2014

Topic editor: Koen Martens

Desk editor: Charlotte Thionois

Printed versions of all papers are also deposited in the libraries of the institutes that are members of

the EJT consortium: Museum National d’Histoire Naturelle, Paris, France; Botanic Garden Meise,

Belgium; Royal Museum for Central Africa, Tervuren, Belgium; Natural History Museum, Fondon,

United Kingdom; Royal Belgian Institute of Natural Sciences, Brussels, Belgium; Natural History

Museum of Denmark, Copenhagen, De nm ark

16